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These changes are mainly due to increased preload, decreased afterload, and changes in cardiac geometry so that left ventricular systolic dysfunction may develop over time in patients without CVD

Primary antibodies were detected with anti-rabbit HRP-conjugated secondary antibody (1:1000, Jackson Laboratory) or anti-mouse HRP-conjugated secondary antibody (1:1000, Pierce)

It is no longer surprising, therefore, that focal silencing (or ablation) of astrocytes renders neurons inapt to control specific behaviors, causally implicating astrocytes in driving higher-order brain functions 65,66,67,68

The isolation and purification of EVs are complex due to their diversity and the presence of other particles in bodily fluids, which is crucial for maintaining purity and avoiding biological effects from contaminants