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WAT explants can remain viable for days to weeks under appropriate culture conditions, allowing for extended observation of dynamic processes with bioactive compounds or drug responses

Cell type analysis To find an association between lipid intensities distribution across regions and the cellular composition of the brain we used mRNA data derived from 35 regions, representing a subset of 75 regions analyzed for their lipid content

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Surgical sympathectomy or pharmacological ganglionic blockade of BAT in rodent models decreases metabolic markers (lipoprotein lipase activity, thermogenic mitochondrial protein activity, glucose membrane transporter density), increases weight without affecting feeding behaviours and inhibits physiological responses to cold challenges