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(7) Centrifuge for 10 min (12000 rpm, 4C), 150 L of supernatant was loaded into an LC-MS injection vial with a liner tube for LC-MS analysis

We applied this assay to analyze whole blood and plasma samples from 11 heathy donors

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Mycothiol Actinomycetes, such as Mycobacteria, Corynebacteria, and Streptomyces, synthesize the cysteinyl pseudo-disaccharide mycothiol (MSH) as major LMW thiol [47]