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Description
After the PCR reaction, 1 uL of DpnI restriction enzyme was added to the reaction, and the mix was incubated for 2 h at 37 C
However, to our knowledge, there is no analytical method in the literature that determines NAC stability and concentration during its use in cells treatment
To assess superoxide production, DHE staining was performed as previously described [21]
Follow-ups were conducted at 1, 3, 6, and 12 months post-treatment
