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Immunofluorescent staining of cells After treatment, cells was fixed for 15 min using fresh, methanol-free 4% formaldehyde, and then, rinsed thrice with PBS for 5 min each

IL-13 switches on the CCL26 gene to synthesize eotaxin-3, which is overexpressed in vivo in the esophageal transcriptome of EoE (Blanchard et al ., 2007)

Murray, MedImmune Ltd, UK Reviewed by Raja Fayad, University of South Carolina, USA
