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However, the unique power of this technique was revealed in a 2010 study of Mattheyses et al., who used fluorescence polarization microscopy to analyze fluorescence anisotropy of fluorescently tagged specific domains of individual (Mattheyses et al., 2010)

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Activation of the RIPK3MLKL axis results in membrane rupture and release of damage-associated molecular patterns (DAMPs), notably HMGB1, ATP, and nucleic acids, which engage pattern-recognition receptors on antigen-presenting cells, thereby driving costimulatory signaling and type I interferon production

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