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The gold standard for lipid peroxidation is mass spectroscopy for 8-isoprostanes, but 4-hydroxynonenal (4-HNE), malondialdehyde (MDA), and thiobarbituric acid reactive substances (TBARS) are also used

However, both glutathione and ascorbate react appreciably slower with singlet oxygen than tocopherols and, especially, carotenoids (Table 1)

doi: 10.1186/1471-2458-12-204

Cells were then washed 3 times with PBS, then washed with DIPA staining solution for 5 min, followed by glycerol blocking, and then observed using a fluorescence microscope ( 2.7 Statistical analysis Statistical analysis and graph production were performed using GraphPad Prism (GraphPad Software) version 6, and Microsoft Excel was used to perform ANOVA for data analysis