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Cells were then transfected with either the pcDNA3 vector (Invitrogen, Waltham, MA, USA) alone as a negative control or with GSK3 or DYRK1A cDNA using JetOptimus transfection reagent (Polyplus transfection, Illkirch, France) following the manufacturers instructions

Rieger, B

Brains were quickly extracted and sliced (300 m) for whole cell recordings in current-clamp and voltage-clamp mode