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The lysate was clarified by centrifugation at 15,000 nx g for 15 min, protein concentrations were determined using the BCA total protein assay (Pierce), and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten min at 95 C

Evaluated with a hold-out test set containing 168 benzoylated molecules, Bz-MS achieved a median WCS value of 0.74 0.03 (95% CI, n = 168) in predicting spectra for benzoylated molecules, which was significantly improved from the pre-trained model, ESI-MLP (median WCS = 0.53 0.02, 95% CI, n = 168) (Fig

Aortic rings were then pre-contracted with 3 M PE

Moreover, both genetic DPP1 deficiency and pharmacological inhibition reduced neutrophil-induced glomerular microvascular endothelial cell damage (163)