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The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH 2 O and PBS, and then probed with the antibody (R1401-12) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system

When to Seek Medical Advice About B12 and Liver Health If you have been diagnosed with fatty liver disease and subsequent blood tests reveal elevated B12 levels, it is important to discuss these findings with your GP or hepatologist

Pituitary adenomas in childhood, adolescence and young adulthood: presentation, management, endocrine and metabolic outcomes