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Similar content being viewed by others Log in or create a free account to read this content Gain free access to this article, as well as selected content from this journal and more on nature.com or Abbreviations 3-ABA: 3-aminobenzamide BSO: DL-buthionine-( S,R )-sulfoximine cisplatin, CDDP: cis -platinum(II)-diammine dichloride FCS: fetal calf serum GSH: reduced glutathione H 2 O 2 : hydrogen peroxide mAb: monoclonal antibody pAb: polyclonal antibody PARP: poly (ADP-ribose) polymerase PBS: phosphate-buffered saline PCD: programmed cell death PI: propidium iodide PKC: protein kinase C ROS: reactive oxygen species References Wyllie AH, Kerr JF and Currie AR (1980) Cell death: the significance of apoptosis

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To enhance the feasibility of utilizing intratumoral microbiomes in tumor diagnosis and treatment, it is imperative to precisely detect and identify low-abundance microbial populations within tumors, followed by their cultivation for the analysis and verification of their biological functions, thereby revealing microbiota-host interactions in the tumor microenvironment (Fig