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Description
Cells were aliquoted into PCR tubes (50 L volume, 1 million cells/condition) for heating at different temperatures (typically 40-67 C in 3 C increments) in a thermal cycler for 3 minutes

Regulatory effects of NAD+ metabolic pathways on sirtuin activity

Medications and supplements
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dose approximated moderate-use exposure while minimizing maternal sedation or locomotor suppression that could confound maternal care and fetal growth
