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Complex III (cytochrome c reductase/cytochrome bc1 complex) is the central component of the respiratory chain, which transfers electrons from coenzyme Q (transfer from complex I and complex II) to cytochrome c (within complex IV) and contributes to the production of proton gradients (mitochondrial inner membrane)

Following euthanasia, mice were dissected and both femurs and tibias were removed and collected in a 50mL Falcon tube containing ice cold HBSS