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RNAseq library preparation To generate BT474 persister cells for RNAseq, BT474 cells were plated in five 15 cm tissue culture plates per replicate, for three replicates, and were allowed to reach 75% confluency before being treated with 2 M lapatinib for nine days
Some drugs also have immunogenicity and allergy risks
Therefore, we regard our ITC data as relative measurements of both SAM binding and conformational changes
10.1016/j.trsl.2006.12.002 122 SemlowD
