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Since a loss of DAPI signal is also observed with nitrofurantoin, which does not bind to DNA but disperses the nucleoid in a similar manner as Cu 3 -TC-Py, nucleoid fragmentation is a considerably more likely explanation for the dispersed DAPI signal and the loss of protein localisation than competition between DAPI and Cu 3 -TC-Py for the minor groove, although it cannot be excluded that the latter may partially contribute to the reduced DAPI signal

For this reason, Kappa GSTs and mPGES-1 (microsomal ProstaGlandin E-Synthase type 1), one subclass of MAPEGs (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) (Bresell et al., 1)

After wash Triton several times, the coverslips were incubated with the conjugated antibody Phalloidin-iFluor 488 (Abcam, ab176753) in a wet chamber for 1 hour at RT

If you continue to experience adverse reactions or worsening symptoms after B12 supplementation, don't hesitate to discuss alternative options with your healthcare provider