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The histidine imidazole and the N-terminal amine and lysine side chain give GHK a high intrinsic affinity for copper(II), and as the free apo-peptide it has been studied in preclinical and in vitro research as a copper-binding ligand and as a signaling molecule implicated in extracellular-matrix remodeling, collagen and metalloproteinase regulation, and antioxidant and anti-inflammatory gene-expression pathways

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However, the specific metabolic checkpoints of the altered thresholds need further exploration, which is a future research direction

Saleh et al., 2016) sequence was used: TR/TE = 2000/80 ms