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Highlighting the specificity of TGM1, involucrin contains 39 repeats of a 10 amino acid sequence, with each containing three glutamine residues (Eckert and Green, 1986), but only one glutamine residue within this 585-amino acid proteinin the sequence ELPEQ Q VGQP (reactive Q is in bold)is reactive in vitro unless the protein is proteolytically degraded (Simon and Green, 1988)

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Because both molecules are hydrophilic, they are readily soluble in standard aqueous solvents