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Description
This process began with applying a Gaussian blur (sigma = 3) to these combined images, aiding in the more precise visualisation of cellular structures

S.SiddiqueH

After sonication, the lysate was centrifuged (17,000 rpm, 40 min) and the supernatant was purified using a nickel-nitrilotriacetic acid column (HisTrap HP
The dose-response curve showed peak activity at low nanomolar concentrations suggesting high potency
