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Plasma glucagon levels were quantified using the Mercodia Glucagon ELISA kit (10-1281-01, Mercodia)

Additionally, excessive ROS leads to oxidative damage to DNA and proteins, as well as lipid peroxidation of cell membranes, ultimately resulting in cell death (21)

Materials and Methods Study approval Animal procedures complied with the welfare guidelines of the European Community and were ethically approved by the Direction of Veterinary departments of Herault, France (agreement number 34.251) and the Languedoc Roussillon Institutional Animal Care and Use Committee, France (#CE-LR-0818)

One activates beta-3 adrenergic receptors in fat tissue