glutathione column protocol Purification of proteins fused to S-tranferase Expression and Purification of GST

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As the above method, the gene of sod was cloned into pKLH73-pKLH79 and pKLH86-pKLH88 containing different promoters by digestion of Bam HI and Eco RI (Supplementary Table 1

Nature 464 , 13671370 (2010)

This interaction promoted the synthesis of GSH, inhibited ferroptosis, and ultimately drove the progression of RCC