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Flow cytometric analysis The mice were anesthetized, and their livers were first perfused with calcium and magnesium-free buffer (10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid [pH 7.4], 5.4 mM KCl, 120 mM NaCl, 0.5 mM EDTA, 5.6 mM glucose, and 5.0 mM NaHCO 3 ) via the inferior vena cava for 3.5 min at a rate of 7 mL/min and then with the Hanks balanced salt solution (5.4 mM KCl, 140 mM NaCl, 0.44 mM KH 2 PO 4 , 0.34 mM Na 2 HPO 4 , 1.3 mM CaCl 2 , 0.98 mM MgCl 2 6H 2 O, 5.6 mM glucose, and 4.2 mM NaHCO 3 ) containing 0.1% collagenase (032-22364

Cycle structure: Semax runs 2 months on, 2 months off

Yu W, Liu W, Xie D, et al

Other anti-inflammatory peptides such as KPV work through distinct pathways and are frequently studied alongside this compound in inflammatory research designs