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10.1016/j.biopha.2021.112416 20 ChenX.HeH.LongB.WeiB.YangP.HuangX.et al (2023a)

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The antioxidant activity of GSH intermediated via thiol (-SH) of its cysteine residue upon its interaction with ROS or electrophiles and the GSH oxidized to GSSG (oxidized Glutathione), the GSSG will reduce to GSH by Glutathione reductase (GR), the activity of GR is therefore very important to keep the antioxidant power of mammalian cells, while GSH/GSSG ratio is an indicator for redox balance in the cell

The resulting pEarleyGate 202/ 35S::FLAG-GSNOR1 vector was transformed into Agrobacterium tumefaciens strain GV3101(pMP90), which was subsequently used to transform WT plants by floral dipping 62