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However, C4 levels in IBS-D are not significantly correlated to mucosal permeability as assessed by urinary excretion of lactulose/mannitol, and in 23 patients with IBS-D, treatment with colesevelam tended to improve stool consistency but did not alter mucosal permeability as assessed by lactulose/mannitol excretion (173)

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Additionally, other factors like infections, weight loss, smoking, drug abuse, and environmental pollution may also contribute to its development [6]

Coverslips were then gently washed with PBS and fixed with 4% paraformaldehyde containing 1 g/ml Hoechst 33342 for 10 minutes at 37C