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This guide covers laboratory preparation only, under research-use-only terms

doi: 10.3389/fendo.2022.1085656 56 YeWXieTSongYZhouL
Cystine (Cysteine Disulfide) Role in Epigenetics and Oxidative Stress Cystine is formed from the oxidation of the SH (sulfhydryl) of the R-groups of two cysteine molecules which generates a disulfide bond

MurF was inserted into the same vector using 5-BamHI and 3-XhoI sites to get pGEX-EcMurF vector (primers are EcMurF-For/EcMurF-Rev)