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Description
Primary pain in ALS includes neuropathic pain and pain due to spasticity or cramps, whereas secondary pain is mainly nociceptive and may appear as muscle weakness and atrophy progress [83]
Proteins were digested using 1 mg/mL proteinase K and incubating at 37C for 5 hours
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Peripheral blood was the collected using a sterile hematocrit tube from the retro-orbital plexus, allowed to clot on the ice, centrifuged at 3000 rpm for 10 min, and the serum was collected and stored at -20C for further AST, ALT, and MDA analysis
