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The animals had free access to food and water throughout the experiment

TNF--mediated keratinocyte expression and release of matrix metalloproteinase 9: putative mechanism of pathogenesis in Stevens-Johnson syndrome/toxic epidermal necrolysis

doi: 10.3389/fphys.2018.01487

Therefore, to reduce the chance of higher variance in our data and number of animals needed for this study, we have focused only on male mice
