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The NEM-treated fraction was used in determination of the reduced-form of glutathione and glutathione disulfide
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Selige J, Hatzelmann A, Dunkern T
Preparation of water soluble lens proteins Four de-capsulated lenses from each group were homogenized in 600 L of lysis buffer (20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na 2 EDTA, 1 mM EGTA, 1% Triton, 2.5 mM sodium pyrophosphate, 1 mM -glycerophosphate, 1 mM Na 3 VO 4 , 1 g/mL leupeptin, and 2 mM PMSF
