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Conversely, high levels of matrix ROS can activate a supraphysiologic uncoupling protein 2 (UCP2)-dependent increase in proton leak, impairing GSIS (Mailloux et al., 2012)
After incubation at 37 C with shaking (500 rpm) for 1 h., the cells were pelleted at 5,000 x g for 10 min at 4 C, washed once with ice-cold phosphate-buffered saline (PBS, pH 7.4), resuspended in 200 L of cold TE lysis buffer (30 mM TrisHCl, 1 mM EDTA, pH 8.0), and transferred to Lysing matrix B tubes (MP Biomedical)
Jiang F, Jia K, Chen Y, Ji C, Chong X, Li Z, et al
Newby R, Muhamed S, Alty J, Cosgrove J, Jamieson S, Smith S, et al
