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All real-time PCRs were performed three times and all changes in gene expression were reported as multiples of increases relative to the control while GAPDH was used as an internal control and relative gene expression between the experimental groups were determined using MxPro software

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For instance, biotin-cysteamine-Cypate conjugates undergo GSH-triggered hydrolysis, leading to aggregation that enhances both imaging signals and photothermal efficacy [83]

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