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Step 4: Add 90ul TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37C

Taurine influences the levels of proteins such as synapsin 1 and postsynaptic density protein-95, which are crucial in the development of synapses [32]

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All this evidence indicated that active transporter on the abluminal side triggers the release of Glu from cerebrospinal fluid (CSF) into ECs and, concomitantly, facilitative carriers on the luminal membrane mediate Glu efflux from ECs to plasma ( Regarding the specific facilitative transporters for Glu on the luminal side, there are two plausible candidates