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HRP-conjugated secondary antibodies (rabbit IgG: 111035144, mouse IgG: 115035003, 1:3000 dilution in 3% BSA, Jackson ImmunoResearch) and the ECL-2 (Thermo Scientific) or ECL (GE Amersham) chemiluminescent reagent were used to visualize the bands using a Bio-Rad ChemiDoc imaging system running Image Lab V4.0 software

The timing of your S-A-GSH administration drastically impacts how much of the antioxidant actually reaches your bloodstream

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Furthermore, ammonium alters the types of cytokines secreted by dendritic cells, diminishing their ability to activate T cells and further limiting anti-tumor immune responses (144)