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To understand why microglia are so heterogeneous, related to multiple diseases and aging, it would be important to discern how glial cells emerged in our ancestors

It has been suggested that excessive ROS production may lead to an upregulation of oncogene gene and the formation of mutagen compounds, which trigger proatherogenic activity and inflammation

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Cells were subsequently analyzed for germ layer-specific differentiation by immunostaining, the proportion of positive cells was quantified using ImageJ (version 1.53e), and RT-qPCR using ectoderm, mesoderm, and endoderm markers