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Description
The substrate GSH was newly supplied and the synthesized -EC was removed when the reaction solution was exchanged at the end of the individual repeated reactions
Chronically high glucose fuels a flood of free radicals, and over time patients are left with less of the reduced, protective form of GSH and more of the oxidized, exhausted kind a red flag of cellular stress

The specific interaction of dinitrosyl-diglutathionyl-iron complex, a natural NO carrier, with the glutathione transferase superfamily: suggestion for an evolutionary pressure in the direction of the storage of nitric oxide
10.1038/nrn.2017.156 Nat