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For chymotrypsin digestion the alkylated proteins were diluted to 1 M urea with 100 mM Tris and 10 mM CaCl2 buffer pH 8.0 (50 g of protease in 57 l added to 456 l of buffer) and treated with 2.5 g of chymotrypsin made fresh in 1 mM HCl

Peptides have become quite popular since of their therapeutic potentials in treatment of these diseases, like anti-aging, weight reduction and bodybuilding

Skeletal muscle is not only a main component of the locomotor system, but functions also as an endocrine organ by secreting numerous bioactive substances (myokines), including inflammatory factors (IL-6, IL-8, IL-15), brain-derived neurotrophic factor (BDNF), fibroblast growth factor 21, irisin, and extracellular vesicles such as exosomes (Li et al., 2025)

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