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In vitro studies, although useful for clarifying molecular pathways as NF-B suppression, antioxidant enzyme overexpression, sometimes employ elevated, non-physiological concentrations of extracts and may fail to correctly simulate the complex context of the human eye

Suboptimal sperm methylation in terms of quality and quantity (CpG island regions restricted to retained histones) has a negative impact on human embryo blastocyst development [9] Approximately 1015% of histones are retained, but these are not randomly located: they bind to the active part/coding area of DNA and so play an important role in embryonic development [6,9]

Indeed, CB1R function is a crucial player in central inflammatory and redox phenomena, underscoring the potential impact of neurodevelopmental THC exposure on these neurophysiological functions