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These pathways are typically assessed using phosphorylation assays, metabolic flux analysis, and gene expression profiling in adipose and related tissues
Measurement of melatonin profiles, particularly, the timing of the sharp rise in melatonin levels during the biological night, can be used to assess circadian phase which is useful in the diagnosis of sleep/circadian pathology as well as determining the optimal timing of treatment

After that, they are given once a month to keep levels up
