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2 Materials and methods 2.1 Scope Samples declared as containing substances intended to enhance sports performance, such as AAS, other PEDs, or substances used after the AAS cycle to mitigate side effects, were included

For transfection, the cells were cultured until 70% confluency and transfected with the pCMV-2B FLAG-GST expression vector using Lipofectamine 3000 reagent in Opti-MEM (Gibco) medium according to the manufacturers protocol (Invitrogen)

The swelling is generally like a raised small bump which is not painful on touching

Schizont Intracellular parasite stage that undergoes multiple rounds of nuclear division and concerted cytokinesis to form new daughter merozoites