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Focusing on astroglia, we combined the analysis of the pharmacokinetic profile of TAM and its metabolites by liquid chromatography-high resolution-tandem mass spectrometry (LC-HR-MS/MS) with the in vivo quantification of recombination events by quantitative real-time polymerase chain reaction (qRT-PCR) to reveal optimal protocols for cell type-specific DNA recombination

VadK binding sites are essential for growth on propionate To directly assess the function of the phosphohistidine and ATP-binding domains in propionate metabolism, we constructed an isogenic panel of vadK mutants by complementing vadK with a chromosomally integrated copy of vadK encoding mutations in the His422 catalytic residue

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After three injections, tissue was collected 24 h after the last injection, corresponding to 3 d after the first injection