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The slides were washed in water/PBS and then blocked using 5% goat serum in PBS for 1 h at room temperature prior to adding primary antibodies diluted in the blocking buffer

Addressing the Concerns: Are There Any Side Effects

For vegetarians, fortified plant-based products and supplements are vital options
As an example, we recently identified two siblings with Wilson disease who had an autoimmune disorder disposition with elevation of anti-nuclear antibodies (ANA) and anti-smooth muscle antibodies, although the IgG level was in both patients in the normal range (55)