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In line with decreased expression of key enzymes involving -oxidation (Figure 3, A and B), metabolomic analysis demonstrated significantly decreased levels of multiple acyl-carnitine species, without alterations in free carnitine levels (Figure 4, EG)
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Conversely, the groups treated with ZFPG and ZFP + US groups exhibited a significant decrease in cell viability

By integrating these disparate elements, our work emphasises the necessity of a holistic approach to diagnosing, researching, and treating ME/CFS, urging the scientific community to reconsider the diseases complexity and the multifaceted approach required for its study and management
Glutathione, the primary redox buffer in plant cells, is indispensable for maintaining redox equilibrium during cell division, as evidenced by growth defects in rml1 mutants deficient in glutathione biosynthesis ( A dynamic redox cycle involving oscillations in ROS, ascorbate, and glutathione levels regulates progression through specific cell cycle checkpoints (Tognetti et al., 2017)
