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100 L of 50% MeOH(aq) spiked with qualitative internal standards (qIS), 0.125 g/mL nitrodracylic acid and 0.125 g/mL isocaramidine sulfate was added to the extract solution, and then vortexed and incubated on ice for 10 min

Label the vial with the date of reconstitution and the resulting concentration