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Thereafter, the sequencing reaction was performed with the same primers used for amplification and Sanger sequencing was performed (ABI PRISM 3100-Avant Analyser, Applied Biosystems, Foster City, CA) in the following conditions: 1 min at 96 C and 25 cycles of 10 sec at 96 C, 5 sec at 56 C and 4 min at 68 C (with a sensitivity threshold of approximately 25%) 91

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However, its essential to acknowledge the limitations of our current hypothesis
