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This variant ( AT87Q ) was generated by introducing the codon for a specific amino-acid residue present in both the gamma-and delta-globin proteins (Q, glutamine) to replace the corresponding codon in beta-globin (T, Threonine) [147]

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To ensure the absorbance from oil red O staining was within the linear detection range of the plate reader, a calibration curve was established for oil red O staining in adipocytes using a previously described protocol.[23] 2.12