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Unfolding of mucins is dependent on concentrations of bicarbonate, calcium, and sodium in the airway with high concentrations of extracellular calcium, acidic pH, or hyperabsorption of sodium preventing unfolding of the gel-forming mucins (Kesimer et al., 2010

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Researchers working with both compounds simultaneously must track two separate reconstitution concentrations and two separate dosing volumes, which adds complexity compared to single-compound research

For each animal 3 10 6 HT-29 cells were mixed 1:1 v/v with LDEV-free extracellular matrix protein Geltrex (Gibco, # A1413201) and inoculated by subcutaneous injection in the right flank