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l carnitina beneficii Suplimente Acetyl L-Carnitina Administrare L-carnitina: rol, beneficii si deficit
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The samples were then placed in a VACUETTE tube and stored at a temperature of 4 C for a duration of 30 min

After preparation, all prepared media were immediately sterilized by autoclaving at 121 for 30 min
4B, right)

For immunofluorescence, sections were washed with PBS at room temperature twice for 10 min, followed by incubation in blocking buffer (5% fish gelatine, 0.5% Triton in PBS) at room temperature for 1 h before incubation with the primary antibodies (see Supplementary Table 4 for primary antibodies used in this study) diluted in the blocking buffer at 4 C overnight
