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Local resolution was estimated for each reconstruction using the RELION implementation of local resolution algorithm 35 , and locally scaled maps were used for model building and refinement in all cases

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Secondary outcome measurements included reduction in body weight from baseline by at least 5% or 10% by week 26, changes in waist circumference, total cholesterol, HDL, LDL, VLDL, and triglycerides, and levels of different glycemic variables including HbA1C, fasting plasma glucose, fasting insulin, insulin resistance, and the functioning of beta-cells

When injected subcutaneously, Tesamorelin binds GHRH receptors in the brains pituitary and triggers a cascade: the pituitary pumps out more endogenous growth hormone, which in turn boosts blood levels of insulin-like growth factor 1 (IGF-1)