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Immunoprecipitation and western blotting Cells were lysed with RIPA buffer, and 500 g whole cell lysate was precipitated with 2 g agarose-conjugated antibody against Flag (Flag beads) and normal IgG (as a negative control)
In the molecular docking experiments, ETS1s 3D structure was used as the receptor, and the GPX4 promoter sequence was modeled as the ligand
Solovyev 1 1 Federal State Research Institution (FSRI) named after V
Cureus 14 , e25384 (2022)
