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Regulation of leukocyte tricarboxylic acid cycle in drug-naive Bipolar Disorder

Elevated levels of intact peroxide antimalarials were observed in resistant parasites over the duration of the incubation, corresponding to a slower rate of activation compared to sensitive parasites

RNA (300 ng) and reverse transcribed with SuperScript III (Invitrogen, Carlsbad, CA) using 150 ng of random hexamers, 10 mM deoxynucleoside triphosphates (dNTPs), and 40 U of RNaseOut (Invitrogen)

This article breaks down each method, compares their strengths and limitations, and outlines best practices for sample handling that prevent artifactual oxidation from compromising data integrity