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The pellet and supernatant were re-suspended in an SDS-PAGE sample buffer and all samples were electrophoresed on 12% denaturing gel at a constant voltage
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When pretreating the cells with N -ethylmaleimide (NEM, 20 mM), a trapping reagent of thiol species, for 0.5 h, the signals from the cytoplasmic regions are decreased in both green and yellow channels (Figure 7DF)
Peng, Y.-F., Wang, L.-L., Gu, J.-H., & Zeng, Y.-Q
