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After fixation, samples underwent blocking through incubation with 5% goat serum with 0.3% Triton X-100 in PBS for 30 min

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The intestinal mucosa is constantly exposed to oxidants and carcinogens taken in from diet and/or bacteria, whose chronic exposure may cause production of free radicals leading to redox imbalance and subsequent DNA damage, disturbing the intestinal metabolic equilibrium (73)

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